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Image Search Results
Journal: Nature Communications
Article Title: Rapid evolution of A(H5N1) influenza viruses after intercontinental spread to North America
doi: 10.1038/s41467-023-38415-7
Figure Lengend Snippet: A , B Solid-phase binding of A(H5N1) viruses to biotinylated sialylglycopolymers A 3’-SialLacNAc-PAA-biotin (3’-SLN) or B 6’-SialLacNAc-PAA-biotin (6’-SLN), representing galactose-linked sialic acids α2,3-SA (the avian virus preferred receptor) and α2,6-SA (the human virus preferred receptor), respectively. The data are shown as the mean ± SD from duplicate wells and representing one of two independent experiments. C Kinetics of pH inactivation of the Wigeon/SC/21, Eagle/FL/22, and CA/04 (H1N1)pdm09 viruses at 37 °C. The data are shown as the mean ± SD from triplicate wells representing one of three independent experiments. D Minireplicon polymerase activities of Wigeon/SC/21 and Eagle/FL/22 at 37 °C. The data are shown as the mean ± SD of 3-4 measurements over the hypothesized pH range for avian viruses, and 2 measurements over previously described ranges for control virus CA/04 (H1N1)pdm09, and representing one of three independent experiments. NS = not significant as determined by paired, two-tailed t -test. E Viral replication kinetics in Calu-3 cells. Cells were inoculated at an MOI of 0.001 and incubated at 37 °C. The data are shown as the mean ± SD from triplicate wells and representing one of two independent experiments. F Viral replication kinetics in primary differentiated human airway cultures. Cultures were inoculated at an MOI of 0.005 and incubated at 37 °C. The data are shown as the mean ± SD from triplicate culture inserts and representing one of two independent experiments. The number of inserts with viral replication out of the total is indicated in parentheses. Statistical significance (one-way ANOVA) was determined by comparison to rg-A/Texas/71/2017 (H3N2) at 72hpi. G , H Neutralizing antibody levels in human serum samples ( n = 48) against HA protein (as measured by HI assay, dotted line indicates limit of detection of 1:10 serum dilution) or NA protein (as measured by ELLA assay). Points joined by lines represent values for the same individual for the individual antigens tested. * P < 0.05, *** P < 0.001, * * ** P < 0.0001.
Article Snippet: Serial sections were subjected to antigen retrieval for 30 min at 98 °C before undergoing immunohistochemical labeling of viral antigen, using a primary goat polyclonal antibody (
Techniques: Binding Assay, Virus, Control, Two Tailed Test, Incubation, Comparison, HI Assay
Journal: Nature Communications
Article Title: Rapid evolution of A(H5N1) influenza viruses after intercontinental spread to North America
doi: 10.1038/s41467-023-38415-7
Figure Lengend Snippet: Susceptibility of North American HPAI A(H5N1) clade 2.3.4.4b to approved antiviral drugs
Article Snippet: Serial sections were subjected to antigen retrieval for 30 min at 98 °C before undergoing immunohistochemical labeling of viral antigen, using a primary goat polyclonal antibody (
Techniques: Virus